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Table 3 Comparisons of GH response in liver and in primary hepatocytes

From: Exploring hepatic hormone actions using a compilation of gene expression profiles

Genes

in vivo

hepatocytes

 

Array

RT-PCR

Array

RT-PCR

Phyh

0.34

0.6

0.54

0.6

Hsd11β1

0.31

0.28

0.63

0.5

Ppp3ca

1.33

1.43

1.48

1.33

CD36

2.39

2.78

1.26

2.07

  1. Microarray and RT-PCR expression measurements for phytanoyl-CoA hydroxylase (Phyh), hydroxysteroid 11-beta dehydrogenase 1 (Hsd11β1), the catalytic subunit of protein phosphatase 3, alpha isoform (Ppp3cα) and fatty acid translocase/CD36 antigen (FAT/CD36). The real-time PCR results were normalized to GAPDH. The ratios between treated and untreated samples in vivo (young male rats treated with bGH or vehicle for 1 week via minipumps, n = 4 per group), and the ratios between GH-treated (24 hours) and untreated primary hepatocytes are shown.
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BMC Physiology

ISSN: 1472-6793

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