Figure 2

HCO ₃ ^--induced up-regulation of sAC protein expression. Cells were starved for HCO₃^- in HCO₃^--free DMEM for 48 hours at 37°C, followed by 24-hour incubation in DMEM with different [HCO₃^-] at 37°C. Cell lysates (60 μg/lane) were separated and transferred to a polyvinylidene fluoride (PVDF) membrane. The membrane was probed with mouse anti-human monoclonal sAC primary antibody, then rinsed and reprobed using mouse anti-human β-actin antibody. A: blots of sAC protein expression and β-actin from BCECs exposed to 0, 10 or 40 mM HCO₃^-. Band density of sAC expression shown in the bar graph is relative to 0 HCO₃^-. B: blots of sAC protein expression and β-actin from Calu-3 cells exposed to 0 and 40 mM HCO₃^-. Band density of sAC expression shown in the bar graph is relative to 0 HCO₃^-. Error bar indicates ± SE. *different from 0 HCO₃^- (n = 3, p < 0.05).