Skip to main content

Table 1 Rankings of the four tested control genes for human athletes produced by geNorm and NormFinder for each of the three groups of samples and for the overall analysis

From: Athletic humans and horses: Comparative analysis of interleukin-6 (IL-6) and IL-6 receptor (IL-6R) expression in peripheral blood mononuclear cells in trained and untrained subjects at rest

 

Sedentary

Low-level athletes

High-level athletes

Total

 

geNorm

(M value)

NormFinder

(SD value)

geNorm

(M value)

NormFinder

(SD value)

geNorm

(M value)

NormFinder

(SD value)

geNorm

(M value)

NormFinder

(SD value)

1

HPRT1/ACT-B*

(0.28)

HPRT1

(0.22)

HPRT1/ACT-B*

(0.24)

HPRT1

(0.12)

HPRT1/ACT-B*

(0.31)

HPRT1

(0.15)

ACT-B/HCYPB*

(0.47)

ACT-B

(0.13)

2

 

HCYPB

(0.31)

 

ACT-B

(0.13)

 

ACT-B

(0.16)

 

HCYPB

(0.41)

3

HCYPB

(0.40)

ACT-B

(0.32)

HCYPB

(0.30)

HCYPB

(0.36)

HCYPB

(0.37)

HCYPB

(0.16)

HPRT1

(0.54)

HPRT1

(0.53)

4

GAPDH

(0.53)

GAPDH

(0.60)

GAPDH

(0.41)

GAPDH

(0.49)

GAPDH

(0.44)

GAPDH

(0.18)

GAPDH

(0.65)

GAPDH

(0.65)

  1. *Denotes the two most stable genes that constitute a couple that cannot be further ranked (Vandesompele et al., 2002). M values (calculated according to the geNorm algorithmic procedures; Vandesompele et al., 2002) and SD values (calculated according to the NormFinder algorithmic procedures; Andersen et al., 2004) constitute different measures of the stability of gene expression across samples.